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Product Info Summary
| SKU: | A10469-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-IMP4 Antibody Picoband™
SKU/Catalog Number
A10469-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-IMP4 Antibody Picoband™ catalog # A10469-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IMP4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10469-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human PCF11 recombinant protein (Position: N1214-V1555).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A10469-1 is reactive to Imp4 in Human, Mouse, Rat
Applications
A10469-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
33.656kDa
Background of Imp4
U3 small nucleolar ribonucleoprotein protein IMP4 is a protein that in humans is encoded by the IMP4 gene. The protein encoded by this gene, along with IMP3 and MPP10, is part of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. This complex is necessary for the early cleavage steps of pre-18S ribosomal RNA processing. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IMP4 using anti-IMP4 antibody (A10469-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse EL-4 whole cell lysates,
Lane 7: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP4 antigen affinity purified polyclonal antibody (Catalog # A10469-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP4 at approximately 36 kDa. The expected band size for IMP4 is at 34 kDa.
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Figure 2. IF analysis of IMP4 using anti-IMP4 antibody (A10469-1) and anti-Beta Tubulin antibody (M01857-3).
IMP4 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IMP4 Antibody (A10469-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-IMP4 antibody (A10469-1).
Overlay histogram showing HepG2 cells stained with A10469-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMP4 Antibody (A10469-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Imp4 (Source: Uniprot.org, NCBI)
Gene Name
Imp4
Full Name
U3 small nucleolar ribonucleoprotein protein IMP4
Weight
33.656kDa
Alternative Names
Brix domain-containing protein 4; BXDC4MGC19606; IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast); U3 small nucleolar ribonucleoprotein protein IMP4; U3 snoRNP protein 4 homolog; U3 snoRNP protein IMP4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Imp4, check out the Imp4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Imp4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IMP4 Antibody Picoband™ (A10469-1)
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