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Product Info Summary
| SKU: | A09441-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Importin subunit alpha-6 KPNA5 Antibody
SKU/Catalog Number
A09441-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Importin subunit alpha-6 KPNA5 Antibody (Catalog # A09441-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
KPNA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Importin subunit alpha-6 KPNA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09441-1)
Host
Rabbit
Contents
KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KPNA5 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human KPNA5. The immunogen is located within amino acids 50 - 100 of KPNA5.
Cross-reactivity
KPNA5 antibody is predicted to not cross-react with other Importin alpha family members.
Reactive Species
A09441-1 is reactive to KPNA5 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
60.3 kDa
Background of KPNA5
Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09441-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of KPNA5 in EL4 cells with KPNA5 antibody at 5 μg/mL.
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Immunofluorescence of KPNA5 in EL4 cells with KPNA5 antibody at 20 μg/mL.
Specific Publications For Anti-Importin subunit alpha-6 KPNA5 Antibody (A09441-1)
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