Product Info Summary
SKU: | A00447-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Insulin Receptor/INSR Antibody Picoband™
SKU/Catalog Number
A00447-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin Receptor/INSR Antibody Picoband™ catalog # A00447-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Insulin Receptor/INSR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00447-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Insulin Receptor/INSR recombinant protein (Position: E168-N621).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00447-2 is reactive to INSR in Human, Mouse, Rat
Applications
A00447-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
156 kDa
Calculated molecular weight
156.333kDa
Background of INSR
INSR(INSULIN RECEPTOR) is a tetramer of 2 alpha and 2 beta subunits that are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. It belongs to the large class of tyrosine kinase receptors. The insulin receptor gene is mapped to 19p13.2. The insulin receptor mediates their activity by causing the addition of a phosphate group to particular tyrosines on certain proteins within a cell. The INSR gene spans more than 120 kb and has 22 exons. Functional studies of the INSR SNPs show no effect on mRNA levels or splicing in peripheral blood leukocytes or on binding of insulin to mononuclear cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Insulin Receptor/INSR using anti-Insulin Receptor/INSR antibody (A00447-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor/INSR antigen affinity purified polyclonal antibody (Catalog # A00447-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor/INSR at approximately 156 kDa. The expected band size for Insulin Receptor/INSR is at 156 kDa.
Protein Target Info & Infographic
Gene/Protein Information For INSR (Source: Uniprot.org, NCBI)
Gene Name
INSR
Full Name
Insulin receptor
Weight
156.333kDa
Superfamily
protein kinase superfamily
Alternative Names
CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; insulin receptor; IR INSR CD220, HHF5 insulin receptor insulin receptor|IR
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INSR, check out the INSR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INSR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin Receptor/INSR Antibody Picoband™ (A00447-2)
Hello CJ!
A00447-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of E2F8 as a Transcriptional Regulator of Gluconeogenesis in Primary Mouse Hepatocytes
Insulin suppresses the expression and function of breast cancer resistance protein in primary cultures of rat brain microvessel endothelial cells
Insulin regulates P-glycoprotein in rat brain microvessel endothelial cells via an insulin receptor-mediated PKC/NF-κB pathway but not a PI3K/Akt pathway
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Customer Reviews
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