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Product Info Summary
| SKU: | M08212 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Insulin Receptor R INSRR Monoclonal Antibody
SKU/Catalog Number
M08212
BM5358 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Insulin Receptor R INSRR Monoclonal Antibody catalog # M08212. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Receptor R INSRR Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08212)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADOI-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Insulin Receptor R
Reactive Species
M08212 is reactive to INSRR in Human, Mouse, Rat
Observed Molecular Weight
80 kDa
Calculated molecular weight
143.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08212 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Insulin Receptor R expression in Hela cell lysate.
Specific Publications For Anti-Insulin Receptor R INSRR Monoclonal Antibody (M08212)
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Customer Q&As
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7 Customer Q&As for Anti-Insulin Receptor R INSRR Monoclonal Antibody
Question
I was wanting to use your anti-Insulin Receptor R Monoclonal antibody for WB for human kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human kidney identification?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
You can see on the product datasheet, M08212 anti-Insulin Receptor R Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-20
Question
Will M08212 anti-Insulin Receptor R Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can see on the product datasheet, M08212 anti-Insulin Receptor R Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-17
Question
Would anti-Insulin Receptor R Monoclonal antibody M08212 work on bovine WB with kidney?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not tested anti-Insulin Receptor R Monoclonal antibody M08212 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Insulin Receptor R Monoclonal antibody M08212 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Is a blocking peptide available for product anti-Insulin Receptor R Monoclonal antibody (M08212)?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
We do provide the blocking peptide for product anti-Insulin Receptor R Monoclonal antibody (M08212). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-07-27
Question
I see that the anti-Insulin Receptor R Monoclonal antibody M08212 works with WB, what is the protocol used to produce the result images on the product page?
L. Edwards
Verified customer
Asked: 2017-11-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-11-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-Insulin Receptor R Monoclonal antibody M08212. Let me know if you need anything else.
P. Rodriguez
Verified customer
Asked: 2014-08-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-27
Question
We are currently using anti-Insulin Receptor R Monoclonal antibody M08212 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
A. Lewis
Verified customer
Asked: 2014-05-01
Answer
The anti-Insulin Receptor R Monoclonal antibody (M08212) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-01
