Anti-Integrin alpha 4 ITGA4 Antibody

Product Name

Anti-Integrin alpha 4 ITGA4 Antibody

View all ITGA4 Antibodies

SKU/Catalog Number

A00468

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Integrin alpha 4 ITGA4 Antibody (Catalog # A00468). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Integrin alpha 4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Integrin alpha 4 ITGA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00468)

Host

Rabbit

Contents

Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Integrin alpha 4 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Integrin alpha 4. The immunogen is located within the last 50 amino acids of Integrin alpha 4.

Cross-reactivity

At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin alpha4 recombinant protein fragment and thus will not recognize it.

Reactive Species

A00468 is reactive to ITGA4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

114.9 kDa

Background of ITGA4

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00468 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ITGA4

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