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- Table of Contents
ITGA4 antibody (PB9648) performs well in Flow Cytometry, IHC, IHC-F, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody (PB9648).
Overlay histogram showing THP-1 cells stained with PB9648 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 4 Antibody (PB9648,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with PB9648 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 4 Antibody (PB9648,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Integrin alpha 4/ITGA4 Antibody Picoband®
ITGA4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | Flow Cytometry, IHC, IHC-F, WB |
Tested Applications
ITGA4 antibody was positively detected by WB in:
Rat Liver Tissue, Rat Kidney Tissue, JURKAT Whole Cell, 22RV1 Whole Cell, HEPG2 Whole Cell, SMMC Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ITGA4 antibody was positively detected by IHC in:
human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ITGA4 antibody was positively detected by IHC-F in:
human placenta tissue
ITGA4 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Human
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Integrin alpha 4/ITGA4 Antibody Picoband®
View all ITGA4 Antibodies
View all ITGA4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha 4, different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
220 kDa
Calculated Molecular Weight
114.9 kDa
Background of ITGA4
ITGA4/Integrin alpha4 is an integrin alpha subunit. It makes up half of the α4β1 lymphocyte homing receptor. The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. ITGA4 is also mapped to 2q31-q32 by fluorescence in situ hybridization.
Cite This Product
Anti-Integrin alpha 4/ITGA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9648)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Dog
97%
High Homology
Cow
93%
High Homology
Rabbit
90%
High Homology
Zebrafish
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ITGA4 antibody
Immunohistochemistry (IHC) protocol for ITGA4 antibody
Flow Cytometry (FC) protocol for ITGA4 antibody
Publications
Application:
Species:
Enhanced neuroprotection in Parkinson's disease by neutrophil-biomimetic nanovesicles through autophagy inhibition and apoptosis antagonism
Published: 2025-08-06
Journal: CHEMICAL ENGINEERING JOURNAL
Application: WB,IF
Impact Factor: 13.20
Species: Mouse
Artificial mesenchymal stem cell extracellular vesicles enhanced ischemic stroke treatment through targeted remodeling brain microvascular endothelial cells
Published: 2025-06-12
Journal: Acta Pharmaceutica Sinica B
Application: WB
Impact Factor: 14.60
Species: Mouse
Enhanced Antiglioma Effect by a Vitamin D3-Inserted Lipid Hybrid Neutrophil Membrane Biomimetic Multimodal Nanoplatform
Published: 2024-12-18
Journal: ACS Nano
Application: WB
Impact Factor: 15.80
Species: Mouse
PMID: 39696957
Reduction of Oxidative Stress and Excitotoxicity by Mesenchymal Stem Cell Biomimetic Co-Delivery System for Cerebral Ischemia-Reperfusion Injury Treatment
Published: 2024-07-01
Journal: Small
Application: WB
Impact Factor: 13.00
Species: Mouse
PMID: 38948959
Biomimetic, folic acid-modified mesoporous silica nanoparticles with “stealth” and “homing” capabilities for tumor therapy
Published: 2024-03-31
Journal: MATERIALS & DESIGN
Application: WB
Impact Factor: 7.60
Species: Mouse,Human
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Product Promise
We promise Anti-Integrin alpha 4/ITGA4 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9648
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026