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Product Info Summary
| SKU: | A01693 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Integrin alpha 6/ITGA6 Antibody Picoband®
SKU/Catalog Number
A01693
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Integrin alpha 6/ITGA6 Antibody Picoband® catalog # A01693. Tested in Flow Cytometry, ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha 6/ITGA6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01693)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Integrin alpha 6/ITGA6 recombinant protein (Position: Q189-K917).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01693 is reactive to ITGA6 in Human, Mouse, Rat
Observed Molecular Weight
127 kDa
Calculated molecular weight
126.6 kDa
Background of ITGA6
Integrin alpha-6, also called VLA-6 or ITGA6B is a protein that in humans is encoded by the ITGA6 gene. This gene is mapped to 2q31.1. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01693 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A431 whole cell, human A549 whole cell, rat kidney tissue, mouse kidney tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of ITGA6 using anti-ITGA6 antibody (A01693).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 antigen affinity purified polyclonal antibody (Catalog # A01693) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA6 at approximately 127 kDa. The expected band size for ITGA6 is at 127 kDa.
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Flow Cytometry analysis of HepG2 cells using anti-ITGA6 antibody (A01693).
Overlay histogram showing HepG2 cells stained with A01693 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGA6 Antibody (A01693, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-Integrin alpha 6/ITGA6 Antibody Picoband® (A01693)
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1 Customer Q&As for Anti-Integrin alpha 6/ITGA6 Antibody Picoband®
Question
We are currently using anti-Integrin alpha 6/ITGA6 antibody A01693 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-Integrin alpha 6/ITGA6 antibody (A01693) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
