Anti-Integrin alpha-9/ITGA9 Antibody Picoband™

Product Name

Anti-Integrin alpha-9/ITGA9 Antibody Picoband™

View all Integrin alpha 9 Antibodies

SKU/Catalog Number

A06851-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Integrin alpha-9/ITGA9 Antibody Picoband™ catalog # A06851-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Integrin alpha-9/ITGA9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06851-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human Integrin alpha-9/ITGA9 recombinant protein (Position: Q526-Q1035). Human ITGA9 shares 89.6% amino acid (aa) sequence identity with mouse ITGA9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06851-1 is reactive to ITGA9 in Human

Applications

A06851-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

114.489kDa

Background of Integrin alpha 9

Integrin alpha-9 is a protein that in humans is encoded by the ITGA9 gene. This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Integrin Alpha-9/ITGA9 using anti-Integrin Alpha-9/ITGA9 antibody (A06851-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha-9/ITGA9 antigen affinity purified polyclonal antibody (Catalog # A06851-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha-9/ITGA9 at approximately 120 kDa. The expected band size for Integrin Alpha-9/ITGA9 is at 115 kDa.

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Figure 2. Flow Cytometry analysis of SH-SY5Y cells using anti-Integrin Alpha-9/ITGA9 antibody (A06851-1).
Overlay histogram showing SH-SY5Y cells stained with A06851-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Alpha-9/ITGA9 Antibody (A06851-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ITGA9 (Source: Uniprot.org, NCBI)

Alternative Names

ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha ITGA9 ALPHA-RLC, ITGA4L, RLC integrin subunit alpha 9 integrin alpha-9|integrin alpha-RLC

For more info on ITGA9, check out the ITGA9 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ITGA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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