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Product Info Summary
| SKU: | A06482 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-IQSEC1 Antibody
SKU/Catalog Number
A06482
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IQSEC1 Antibody (Catalog # A06482). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IQSEC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-IQSEC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06482)
Host
Rabbit
Contents
IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1. The immunogen is located within amino acids 60 - 110 of IQSEC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.
Reactive Species
A06482 is reactive to IQSEC1 in Human, Mouse, Rat
Applications
A06482 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
108.314kDa
Background of IQSEC1
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.
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Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.
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Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.
Red: IQSEC1 Antibody (A06482)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For IQSEC1 (Source: Uniprot.org, NCBI)
Gene Name
IQSEC1
Full Name
IQ motif and SEC7 domain-containing protein 1
Weight
108.314kDa
Superfamily
BRAG family
Alternative Names
ADP-ribosylation factors guanine nucleotide-exchange protein 100; ADP-ribosylation factors guanine nucleotide-exchange protein 2; ARFGEP100; BRAG2; IQ motif and Sec7 domain 1; IQ motif and SEC7 domain-containing protein 1; KIAA0763Brefeldin-resistant Arf-GEF 2 protein IQSEC1 ARF-GEP100, ARFGEP100, BRAG2, GEP100, IDDSSBA IQ motif and Sec7 domain ArfGEF 1 IQ motif and SEC7 domain-containing protein 1|ADP-ribosylation factors guanine nucleotide-exchange protein 100|ADP-ribosylation factors guanine nucleotide-exchange protein 2|IQ motif and Sec7 domain 1|brefeldin A-resistant ARF-GEF2|brefeldin-resistant Arf-GEF 2 protein|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IQSEC1, check out the IQSEC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IQSEC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IQSEC1 Antibody (A06482)
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No publications found for A06482
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Customer Reviews
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