Anti-IRAK IRAK1 Monoclonal Antibody [8F1A7]

Product Name

Anti-IRAK IRAK1 Monoclonal Antibody [8F1A7]

View all IRAK1 Antibodies

SKU/Catalog Number

M01021-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IRAK IRAK1 Monoclonal Antibody [8F1A7] (Catalog # M01021-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

IRAK monoclonal antibody can be stored at -20°C, stable for one year.

Cite This Product

Anti-IRAK IRAK1 Monoclonal Antibody [8F1A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01021-1)

Host

Mouse

Contents

IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 8F1A7

Isotype

IgG1

Immunogen

Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.

Reactive Species

M01021-1 is reactive to IRAK1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

76.5 kDa

Background of IRAK1

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01021-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IRAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information IRAK1

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