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Product Info Summary
| SKU: | M08908 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9)
SKU/Catalog Number
M08908
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) catalog # M08908. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08908)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
2E7D9
Isotype
IgG1
Immunogen
E.coli-derived human IRBIT/AHCYL1 recombinant protein (Position: E14-K57).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M08908 is reactive to AHCYL1 in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.0 kDa
Background of AHCYL1
Putative adenosylhomocysteinase 2 is an enzyme that in humans is encoded by the AHCYL1 gene. The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08908 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, rat brain tissue, rat pancreas tissue, mouse brain tissue, mouse pancreas tissue
FCM: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (M08908).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IRBIT/AHCYL1 antigen affinity purified monoclonal antibody (Catalog # M08908) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 59 kDa. The expected band size for IRBIT/AHCYL1 is at 59 kDa.
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Flow Cytometry analysis of K562 cells using anti-IRBIT/AHCYL1 antibody (M08908).
Overlay histogram showing K562 cells stained with M08908 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRBIT/AHCYL1 Antibody (M08908, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) (M08908)
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