Click image to see more details
Product Info Summary
| SKU: | M00958-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IRF5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00958-1
BM4643 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRF5 Rabbit Monoclonal Antibody catalog # M00958-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRF5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00958-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GGD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRF5
Reactive Species
M00958-1 is reactive to IRF5 in Human
Observed Molecular Weight
56 kDa
Calculated molecular weight
56.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00958-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of IRF5 expression in Ramos cell lysate.
Specific Publications For Anti-IRF5 Rabbit Monoclonal Antibody (M00958-1)
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5 Customer Q&As for Anti-IRF5 Rabbit Monoclonal Antibody
Question
I see that the anti-IRF5 Rabbit Monoclonal antibody M00958-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-26
Question
Will anti-IRF5 Rabbit Monoclonal antibody M00958-1 work on bovine WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
Our lab technicians have not tested anti-IRF5 Rabbit Monoclonal antibody M00958-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IRF5 Rabbit Monoclonal antibody M00958-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cortex of kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-26
Question
Do you have a BSA free version of anti-IRF5 Rabbit Monoclonal antibody M00958-1 available?
T. Jones
Verified customer
Asked: 2017-07-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF5 Rabbit Monoclonal antibody M00958-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-18
Question
See attached the WB image, lot number and protocol we used for cortex of kidney using anti-IRF5 Rabbit Monoclonal antibody M00958-1. Please let me know if you require anything else.
J. Moore
Verified customer
Asked: 2015-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-24
Question
Would anti-IRF5 Rabbit Monoclonal antibody M00958-1 work for WB with cortex of kidney?
F. Parker
Verified customer
Asked: 2013-05-02
Answer
According to the expression profile of cortex of kidney, IRF5 is highly expressed in cortex of kidney. So, it is likely that anti-IRF5 Rabbit Monoclonal antibody M00958-1 will work for WB with cortex of kidney.
Boster Scientific Support
Answered: 2013-05-02
