Anti-IRF5 Rabbit Monoclonal Antibody

IRF5 antibody

Boster Bio Anti-IRF5 Rabbit Monoclonal Antibody catalog # M00958-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00958-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-IRF5 Rabbit Monoclonal Antibody

View all IRF5 Antibodies

SKU/Catalog Number

M00958-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IRF5 Rabbit Monoclonal Antibody catalog # M00958-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRF5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00958-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GGD-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IRF5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00958-1 is reactive to IRF5 in Human

Applications

M00958-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

56.044kDa

Background of IRF5

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For IRF5 (Source: Uniprot.org, NCBI)

Gene Name

IRF5

Full Name

Interferon regulatory factor 5

Weight

56.044kDa

Superfamily

IRF family

Alternative Names

IBD14; interferon regulatory factor 5; IRF5; IRF-5; SLEB10 IRF5 SLEB10 interferon regulatory factor 5 interferon regulatory factor 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IRF5, check out the IRF5 Infographic

IRF5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IRF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-IRF5 Rabbit Monoclonal Antibody

Question

I see that the anti-IRF5 Rabbit Monoclonal antibody M00958-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-26

Question

Will anti-IRF5 Rabbit Monoclonal antibody M00958-1 work on bovine WB with cortex of kidney?

Verified Customer

Verified customer

Asked: 2018-07-26

Answer

Our lab technicians have not tested anti-IRF5 Rabbit Monoclonal antibody M00958-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IRF5 Rabbit Monoclonal antibody M00958-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cortex of kidney in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-26

Question

Do you have a BSA free version of anti-IRF5 Rabbit Monoclonal antibody M00958-1 available?

T. Jones

Verified customer

Asked: 2017-07-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IRF5 Rabbit Monoclonal antibody M00958-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-18

Question

See attached the WB image, lot number and protocol we used for cortex of kidney using anti-IRF5 Rabbit Monoclonal antibody M00958-1. Please let me know if you require anything else.

J. Moore

Verified customer

Asked: 2015-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-04-24

Question

Would anti-IRF5 Rabbit Monoclonal antibody M00958-1 work for WB with cortex of kidney?

F. Parker

Verified customer

Asked: 2013-05-02

Answer

According to the expression profile of cortex of kidney, IRF5 is highly expressed in cortex of kidney. So, it is likely that anti-IRF5 Rabbit Monoclonal antibody M00958-1 will work for WB with cortex of kidney.

Boster Scientific Support

Answered: 2013-05-02

Order DetailsPrice
M00958-1

100μL

$370
M00958-1-30ul

30μL sample

$99
M00958-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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Eddy test
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M00958-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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