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Acod1 antibody (M13250) performs well in IF, ICC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of IRG1/ACOD1 using anti-IRG1/ACOD1 antibody (M13250).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human THP-1+LPS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRG1/ACOD1 antigen affinity purified monoclonal antibody (M13250) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRG1/ACOD1 at approximately 54 kDa. The expected band size for IRG1/ACOD1 is at 54 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human THP-1+LPS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRG1/ACOD1 antigen affinity purified monoclonal antibody (M13250) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRG1/ACOD1 at approximately 54 kDa. The expected band size for IRG1/ACOD1 is at 54 kDa.
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Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14)
Acod1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IF, ICC, WB |
Tested Applications
Acod1 antibody was positively detected by WB in:
human THP-1 whole cell lysates, human THP-1+LPS whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunocytochemistry/Immunofluorescence
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14)
View all ACOD1 Antibodies
View all ACOD1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
33A14
Immunogen
Recombinant protein within mouse IRG1 aa 3-486.
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
53.8 kDa
Background of Acod1
Enables aconitate decarboxylase activity and protein homodimerization activity. Involved in several processes, including cellular response to cytokine stimulus; regulation of defense response; and regulation of gene expression. Acts upstream of or within response to lipopolysaccharide. Located in mitochondrion. Orthologous to human ACOD1 (aconitate decarboxylase 1).
Cite This Product
Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13250)
Protocols
Western Blot protocol for Acod1 antibody
Review
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Product Promise
We promise Anti-IRG1/ACOD1 Antibody (Monoclonal, 33A14) performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M13250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026