Anti-IRGM Antibody

Product Name

Anti-IRGM Antibody

View all Irgm Antibodies

SKU/Catalog Number

A03614

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IRGM Antibody (Catalog # A03614). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-IRGM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03614)

Host

Rabbit

Contents

IRGM Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM. The immunogen is located within amino acids 30 - 80 of IRGM.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36.

Reactive Species

A03614 is reactive to Irgm in Human, Mouse, Rat

Applications

A03614 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.338kDa

Background of Irgm

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 μg/ml.

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Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.

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Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 μg/mL.

Green: IRGM Antibody (A03614)
Blue: DAPI staining

Gene/Protein Information For Irgm (Source: Uniprot.org, NCBI)

Alternative Names

IFI1; immunity-related GTPase family M protein; immunity-related GTPase family, M; interferon-inducible protein 1; IRGM1; LPS-stimulated RAW 264.7 macrophage protein 47 homolog; LRG47; LRG-47; LRG-47-like protein; M1; MGC149263; MGC149264 Irgm|Ifggd3, Ifi1|immunity-related GTPase M|immunity-related GTPase family M protein|immunity-related GTPase family, M|interferon inducible protein 1|interferon-gamma-inducible GTPase Ifggd3 protein

For more info on Irgm, check out the Irgm Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Irgm: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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