Anti-IRX2 Antibody

Irx2 antibody

Boster Bio Anti-IRX2 Antibody (Catalog # A12205). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A12205
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-IRX2 Antibody

View all Irx2 Antibodies

SKU/Catalog Number

A12205

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-IRX2 Antibody (Catalog # A12205). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12205)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human IRX2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12205 is reactive to Irx2 in Human

Applications

A12205 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

49.484kDa

Background of Irx2

IRX2 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.[supplied by OMIM, Apr 2004]

Hansen L., Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ogura K., Cytogenet. Cell Genet. 92:320-325(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For Irx2 (Source: Uniprot.org, NCBI)

Gene Name

Irx2

Full Name

Iroquois-class homeodomain protein IRX-2

Weight

49.484kDa

Superfamily

TALE/IRO homeobox family

Alternative Names

Homeodomain protein IRXA2; iroquois homeobox 2; Iroquois homeobox protein 2; iroquois-class homeodomain protein IRX-2; IRXA2 Irx2|IR|Iroquois homeobox 2|iroquois-class homeodomain protein IRX-2|Iroquois related homeobox 2|homeodomain protein IRXA2|iroquois homeobox protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Irx2, check out the Irx2 Infographic

Irx2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Irx2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Find answers in Q&As, reviews.

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7 Customer Q&As for Anti-IRX2 Antibody

Question

We are currently using anti-IRX2 antibody A12205 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

The anti-IRX2 antibody (A12205) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-14

Question

I am interested in to test anti-IRX2 antibody A12205 on human upper lobe of lung for research purposes, then I may be interested in using anti-IRX2 antibody A12205 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

The products we sell, including anti-IRX2 antibody A12205, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for upper lobe of lung using anti-IRX2 antibody A12205. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-20

Question

Does anti-IRX2 antibody A12205 work for IF with upper lobe of lung?

Verified Customer

Verified customer

Asked: 2019-03-29

Answer

According to the expression profile of upper lobe of lung, IRX2 is highly expressed in upper lobe of lung. So, it is likely that anti-IRX2 antibody A12205 will work for IF with upper lobe of lung.

Boster Scientific Support

Answered: 2019-03-29

Question

I see that the anti-IRX2 antibody A12205 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-06-07

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-07

Question

Is a blocking peptide available for product anti-IRX2 antibody (A12205)?

S. Johnson

Verified customer

Asked: 2015-04-22

Answer

We do provide the blocking peptide for product anti-IRX2 antibody (A12205). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-04-22

Question

Will anti-IRX2 antibody A12205 work on horse WB with upper lobe of lung?

O. Anderson

Verified customer

Asked: 2013-06-27

Answer

Our lab technicians have not tested anti-IRX2 antibody A12205 on horse. You can run a BLAST between horse and the immunogen sequence of anti-IRX2 antibody A12205 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse upper lobe of lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-27

Order DetailsPrice
A12205-100uL

100uL

$360

Free Secondary Antibody

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A12205
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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