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Product Info Summary
|Application:||Flow Cytometry, IHC, WB|
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Anti-ITK Monoclonal Antibody
Boster Bio Anti-ITK Monoclonal Antibody catalog # M01385. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ITK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01385)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human ITK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01385 is reactive to ITK in Human
M01385 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ITK expression in Jurkat cell lysate using anti-ITK antibody (M01385).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITK antigen affinity purified polyclonal antibody (Catalog # M01385) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ITK.
Gene/Protein Information For ITK (Source: Uniprot.Org, NCBI)
Tyrosine-protein kinase ITK/TSK
protein kinase superfamily
EC 2.7.10; EC 184.108.40.206; EMT; EMTMGC126257; homolog of mouse T-cell itk/tsk; IL2-inducible T-cell kinase; ITK; Kinase EMT; LYK; LYKMGC126258; PSCTK2; T-cell-specific kinase; Tcsk; tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk ITK EMT, LPFS1, LYK, PSCTK2 IL2 inducible T cell kinase tyrosine-protein kinase ITK/TSK|IL-2-inducible T-cell kinase|T-cell-specific kinase|homolog of mouse T-cell itk/tsk|interleukin-2-inducible T-cell kinase|kinase EMT|tyrosine-protein kinase LYK*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ITK, check out the ITK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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