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Product Info Summary
| SKU: | M01385 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ITK Monoclonal Antibody
SKU/Catalog Number
M01385
BM5205 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ITK Monoclonal Antibody catalog # M01385. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ITK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01385)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACAB-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ITK.
Reactive Species
M01385 is reactive to ITK in Human
Observed Molecular Weight
72 kDa
Calculated molecular weight
71.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01385 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ITK expression in Jurkat cell lysate.
Specific Publications For Anti-ITK Monoclonal Antibody (M01385)
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Customer Q&As
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7 Customer Q&As for Anti-ITK Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01385?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-ITK Monoclonal Antibody (M01385) expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-ITK Monoclonal antibody M01385. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Is a blocking peptide available for product anti-ITK Monoclonal antibody (M01385)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
We do provide the blocking peptide for product anti-ITK Monoclonal antibody (M01385). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-23
Question
What is the immunogen sequence of M01385? Would this antibody target the protein aa395 - 497 region?
Verified customer
Asked: 2019-09-16
Answer
The immunogen sequence of the Anti-ITK Monoclonal Antibody (M01385) is aa1-17 MNNFILLEEQLIKKSQC.
Boster Scientific Support
Answered: 2019-09-17
Question
Do you have a BSA free version of anti-ITK Monoclonal antibody M01385 available?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ITK Monoclonal antibody M01385 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-14
Question
Does M01385 anti-ITK Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
It shows on the product datasheet, M01385 anti-ITK Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-17
Question
Does anti-ITK Monoclonal antibody M01385 work for WB with thymus?
E. Jones
Verified customer
Asked: 2018-02-07
Answer
According to the expression profile of thymus, ITK is highly expressed in thymus. So, it is likely that anti-ITK Monoclonal antibody M01385 will work for WB with thymus.
Boster Scientific Support
Answered: 2018-02-07
