Click image to see more details
Product Info Summary
| SKU: | A01428-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Jagged 2/JAG2 Antibody Picoband®
SKU/Catalog Number
A01428-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Jagged 2/JAG2 Antibody Picoband® catalog # A01428-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Jagged 2/JAG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01428-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human Jagged 2/JAG2 recombinant protein (Position: Q22-N1153). Human JAG2 shares 91.1% and 91% amino acid (aa) sequence identity with mouse and rat JAG2, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01428-1 is reactive to JAG2 in Human
Observed Molecular Weight
130 kDa
Calculated molecular weight
133.4 kDa
Background of JAG2
Protein jagged-2, also known as SER2, is a protein that in humans is encoded by the JAG2 gene. It is mapped to 14q32.33. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Notch ligands Delta1 and JAG2 are subject to presenilin-dependent, intramembranous gamma-secretase processing, resulting in the production of soluble intracellular derivatives. JAG2 can promote the growth and dissemination of uveal melanoma cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01428-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Jagged 2/JAG2 using anti-Jagged 2/JAG2 antibody (A01428-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Jagged 2/JAG2 antigen affinity purified polyclonal antibody (Catalog # A01428-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Jagged 2/JAG2 at approximately 130 kDa. The expected band size for Jagged 2/JAG2 is at 133 kDa.
Specific Publications For Anti-Jagged 2/JAG2 Antibody Picoband® (A01428-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Jagged 2/JAG2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Jagged 2/JAG2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
