Anti-JHD3B KDM4B Antibody

Boster Bio Anti-JHD3B KDM4B Antibody catalog # A04127. Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A04127
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: IHC

Product Name

Anti-JHD3B KDM4B Antibody

See all JMJD2B products

SKU/Catalog Number

A04127

Size

100μl

Form

Liquid

Description

Boster Bio Anti-JHD3B KDM4B Antibody catalog # A04127. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JHD3B KDM4B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04127)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from C-terminal of human JHD3B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04127 is reactive to KDM4B in Human

Applications

A04127 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For KDM4B (Source: Uniprot.Org, NCBI)

Uniprot ID

O94953

Gene ID

23030

Gene Name

KDM4B

Full Name

Lysine-specific demethylase 4B

Weight

121.897kDa

Superfamily

JHDM3 histone demethylase family

Alternative Names

EC 1.14.11; FLJ44906; JHDM3B; JmjC domain-containing histone demethylation protein 3B; JMJD2BEC 1.14.11.-; jumonji domain containing 2B; Jumonji domain-containing protein 2B; KIAA0876lysine-specific demethylase 4B; lysine (K)-specific demethylase 4B KDM4B JMJD2B, TDRD14B lysine demethylase 4B lysine-specific demethylase 4B|[histone H3]-trimethyl-L-lysine(9) demethylase 4B|jmjC domain-containing histone demethylation protein 3B|jumonji domain containing 2B|jumonji domain-containing protein 2B|lysine (K)-specific demethylase 4B|tudor domain containing 14B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KDM4B, check out the KDM4B Infographic

KDM4B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KDM4B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04127

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13 Customer Q&As for Anti-JHD3B KDM4B Antibody

Question

I see that the anti-JHD3B antibody A04127 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-30

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-30

Question

Is a blocking peptide available for product anti-JHD3B antibody (A04127)?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

We do provide the blocking peptide for product anti-JHD3B antibody (A04127). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-06

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-JHD3B antibody A04127. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-27

Question

Would A04127 anti-JHD3B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Patel

Verified customer

Asked: 2019-07-05

Answer

As indicated on the product datasheet, A04127 anti-JHD3B antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-05

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-JHD3B antibody A04127. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-17

Question

I have a question about product A04127, anti-JHD3B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Kulkarni

Verified customer

Asked: 2019-04-01

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04127 anti-JHD3B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-01

Question

Do you have a BSA free version of anti-JHD3B antibody A04127 available?

A. Carter

Verified customer

Asked: 2018-12-27

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-JHD3B antibody A04127 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-27

Question

We are currently using anti-JHD3B antibody A04127 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

The anti-JHD3B antibody (A04127) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-08-06

Question

Would anti-JHD3B antibody A04127 work on primate IHC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2017-09-04

Answer

Our lab technicians have not tested anti-JHD3B antibody A04127 on primate. You can run a BLAST between primate and the immunogen sequence of anti-JHD3B antibody A04127 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukemic t-cell in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-04

Question

Our lab want to know about to test anti-JHD3B antibody A04127 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-JHD3B antibody A04127 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Z. Anderson

Verified customer

Asked: 2017-07-03

Answer

The products we sell, including anti-JHD3B antibody A04127, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-07-03

Question

Is this A04127 anti-JHD3B antibody reactive to the isotypes of KDM4B?

G. Bhatt

Verified customer

Asked: 2017-02-13

Answer

The immunogen of A04127 anti-JHD3B antibody is Synthesized peptide derived from C-terminal of human JHD3B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-02-13

Question

Will anti-JHD3B antibody A04127 work for IHC with cervix carcinoma erythroleukemia?

A. Lewis

Verified customer

Asked: 2014-06-23

Answer

According to the expression profile of cervix carcinoma erythroleukemia, KDM4B is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-JHD3B antibody A04127 will work for IHC with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2014-06-23

Question

I was wanting to use your anti-JHD3B antibody for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

E. Carter

Verified customer

Asked: 2014-05-13

Answer

It shows on the product datasheet, A04127 anti-JHD3B antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-05-13

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A04127
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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