Product Info Summary
SKU: | A03994-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-JMJD1A KDM3A Antibody
View all Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibodies
SKU/Catalog Number
A03994-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD1A KDM3A Antibody (Catalog # A03994-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
JMJD1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD1A KDM3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03994-1)
Host
Rabbit
Contents
JMJD1A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Anti-JMJD1A antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human JMJD1A. The immunogen is located within amino acids 50-100 of JMJD1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody will not cross-react with JMJD1B or JMJD1C.
Reactive Species
A03994-1 is reactive to KDM3A in Human, Rat
Applications
A03994-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
147.341kDa
Background of Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A
The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: JMJD1A, A03994-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: JMJD1A, A03994-1 (2 μg/mL), JMJD1A, (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human HepG2 Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: JMJD1A, A03994-1 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunohistochemistry Validation of JMJD1A in Rat Liver Tissue
Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-JMJD1A antibody (A03994-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunofluorescence Validation of JMJD1A in Rat Liver Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling JMJD1A with A03994-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Protein Target Info & Infographic
Gene/Protein Information For KDM3A (Source: Uniprot.org, NCBI)
Gene Name
KDM3A
Full Name
Lysine-specific demethylase 3A
Weight
147.341kDa
Superfamily
JHDM2 histone demethylase family
Alternative Names
EC 1.14.11; EC 1.14.11.-; JHDM2A; JHDM2AJMJD1AJHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; jumonji C domain-containing histone demethylase 2A; jumonji domain containing 1; jumonji domain containing 1A; Jumonji domain-containing protein 1A; KDM3A; KIAA0742; KIAA0742DKFZp686A24246; Lysine (K)specific Demethylase 3A; Lysine (K)-specific Demethylase 3A; lysine-specific demethylase 3A; testis-specific protein A; TSGA; TSGADKFZp686P07111 KDM3A JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA lysine demethylase 3A lysine-specific demethylase 3A|[histone H3]-dimethyl-L-lysine(9) demethylase 3A|jmjC domain-containing histone demethylation protein 2A|jumonji C domain-containing histone demethylase 2A|jumonji domain-containing protein 1A|lysine (K)-specific demethylase 3A|testis-specific protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM3A, check out the KDM3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JMJD1A KDM3A Antibody (A03994-1)
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No publications found for A03994-1
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