Anti-JMJD3 KDM6B Antibody

Product Name

Anti-JMJD3 KDM6B Antibody

View all Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibodies

SKU/Catalog Number

A01309

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-JMJD3 KDM6B Antibody (Catalog # A01309). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

JMJD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-JMJD3 KDM6B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01309)

Host

Rabbit

Contents

JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Anti-JMJD3 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody is specific for JMJD3 and will not recognize other JMJD proteins.

Reactive Species

A01309 is reactive to KDM6B in Human, Mouse, Rat

Applications

A01309 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

176.632kDa

Background of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3

The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: JMJD3, A01309, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: JMJD3 A01309 (1 μg/mL), JMJD3, (2 μg/mL) and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in HeLa Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: JMJD3, A01309 (0.25 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in K562 Cell Lysate
Loading: 15 μg of lysates per lane. Antibodies: : JMJD3

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Western Blot Validation in Mouse A-20 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: : JMJD3

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Western Blot Validation in HepG2 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: : JMJD3

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Immunocytochemistry Validation of JMJD3 in K562 Cells
Immunocytochemical analysis of K562 cells using anti-JMJD3 antibody (A01309) at 2.5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Immunofluorescence Validation of JMJD3 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with A01309 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Gene/Protein Information For KDM6B (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.11; EC 1.14.11.-; JmjC domain-containing protein 3; JMJD3; JMJD3lysine-specific demethylase 6B; jumonji domain containing 3, histone lysine demethylase; Jumonji domain-containing protein 3; KDM6B; KIAA0346jumonji domain containing 3; Lysine (K)specific Demethylase 6B; Lysine (K)-specific Demethylase 6B; Lysine demethylase 6B KDM6B JMJD3, NEDCFSA lysine demethylase 6B lysine-specific demethylase 6B|[histone H3]-trimethyl-L-lysine(27) demethylase 6B|jmjC domain-containing protein 3|jumonji domain containing 3, histone lysine demethylase|jumonji domain-containing protein 3|lysine (K)-specific demethylase 6B

For more info on KDM6B, check out the KDM6B Infographic

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In this infographic, you will see the following information for KDM6B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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