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Product Info Summary
| SKU: | A16245 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-JMJD4 Antibody
SKU/Catalog Number
A16245
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD4 Antibody (Catalog # A16245). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Rat.
Storage & Handling
JMJD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16245)
Host
Rabbit
Contents
JMJD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JMJD4 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human JMJD4. The immunogen is located within amino acids 130 - 180 of JMJD4.
Cross-reactivity
This antibody will not recognize other members of the JMJD family.
Reactive Species
A16245 is reactive to JMJD4 in Human, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.5 kDa
Background of JMJD4
The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the JMJD4 has not been identified. JMJD4 is expressed in multiple tissues and At least three isoforms of JMJD4 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16245 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JMJD4 antibody can be used for detection of JMJD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 μg/mL.
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Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 μg/mL.
Specific Publications For Anti-JMJD4 Antibody (A16245)
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