Anti-JNK3 Rabbit Monoclonal Antibody

Product Name

Anti-JNK3 Rabbit Monoclonal Antibody

View all JNK3 Antibodies

SKU/Catalog Number

M04297

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-JNK3 Rabbit Monoclonal Antibody catalog # M04297. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JNK3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04297)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HGC-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human JNK3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04297 is reactive to MAPK10 in Human, Mouse, Rat

Applications

M04297 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

50,53 kDa

Calculated molecular weight

52.585kDa

Background of JNK3

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:500

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of JNK3 using anti-JNK3 antibody (M04297).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK3 antigen affinity purified monoclonal antibody (Catalog # M04297) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK3 at approximately 50,53 kDa. The expected band size for JNK3 is at 53 kDa.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

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Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For MAPK10 (Source: Uniprot.org, NCBI)

Alternative Names

c-Jun N-terminal kinase 3; EC 2.7.11; EC 2.7.11.24; JNK3 alpha protein kinase; JNK3; JNK3A; JNK3FLJ12099; MAP kinase 10; MAP kinase p49 3F12; MAPK 10; MAPK10; MGC50974; mitogen-activated protein kinase 10; p493F12; p54bSAPK; PRKM10FLJ33785; SAPK1 beta; stress activated protein kinase beta; Stress-activated protein kinase JNK3 MAPK10 JNK3, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK mitogen-activated protein kinase 10 mitogen-activated protein kinase 10|JNK3 alpha protein kinase|MAP kinase 10|MAP kinase p49 3F12|c-Jun N-terminal kinase 3|stress activated protein kinase beta|stress-activated protein kinase 1b|stress-activated protein kinase JNK3

For more info on MAPK10, check out the MAPK10 Infographic

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In this infographic, you will see the following information for MAPK10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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