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KRT19 antibody (PB9715) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

pb9715 krt19 primary antibodies fcm testing 22

Flow Cytometry analysis of MCF-7 cells using anti-Cytokeratin 19 antibody (PB9715).
Overlay histogram showing MCF-7 cells stained with PB9715 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 19 Antibody (PB9715,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Cytokeratin 19/KRT19 Antibody Picoband®

KRT19 antibody

16 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

KRT19 antibody was positively detected by WB in:

human placenta tissuehuman MCF-7 whole cell, human SW620 whole cell, human HepG2 whole cell, human PANC-1 whole cell, rat lung tissue, rat small intestine tissue, mouse lung tissue, mouse HEPA1-6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

KRT19 antibody was positively detected by IHC in:

human lung cancer tissues, human intestinal cancer tissues, mouse small intestine tissues, rat small intestine tissues, human lung cancer tissues, human bladder cancer tissue, human breast cancer tissue, human cervical cancer tissue, human ovarian cancer tissue, human pancreas cancer tissue, human stomach cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

KRT19 antibody was positively detected by ICC/IF in:

MCF-7 cell

KRT19 antibody was positively detected by IF in:

human colon cancer tissue, human bladder cancer tissue, human breast cancer tissue, human cervical cancer tissue, human ovarian cancer tissue, human pancreas cancer tissue, human stomach cancer tissue

KRT19 antibody was positively detected by FC in:

MCF-7 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Cytokeratin 19/KRT19 Antibody Picoband®
View all KRT19 Antibodies

Form

Lyophilized

Uniprot

P08727

Gene ID

3880

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19, different from the related mouse and rat sequences by nine amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

44 kDa

Calculated Molecular Weight

44.1 kDa

Background of KRT19

Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene. The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Cite This Product

Anti-Cytokeratin 19/KRT19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9715)

Protocols

Western Blot protocol for KRT19 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for KRT19 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for KRT19 antibody

Review Protocol

Immunofluorescence (IF) protocol for KRT19 antibody

Review Protocol

Flow Cytometry (FC) protocol for KRT19 antibody

Review Protocol

Publications

High‐Fat and High‐Sugar Diet Induces Murine Premature Ovarian Failure by Promoting Mitochondrial Oxidative Phosphorylation Response

Published: 2025-09-18

Journal: Food Science & Nutrition

Application: WB,IF

Impact Factor: 3.80

Species: Mouse

PMID: 40979580

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Anti-Cytokeratin 19/KRT19 Antibody Picoband®

KRT19 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

High‐Fat and High‐Sugar Diet Induces Murine Premature Ovarian Failure by Promoting Mitochondrial Oxidative Phosphorylation Response

Silk fibroin–collagen hydrogel loaded with IGF1-CESCs attenuates intervertebral disk degeneration by accelerating annulus fibrosus healing in rats

Piezo1 Regulates Odontogenesis via a FAM83G-Mediated Mechanism in Dental Papilla Cells In Vitro and In Vivo

AHR regulates liver enlargement and regeneration through the YAP signaling pathway

Identification and culture of functional salivary gland ductal epithelial cells

Bile duct hamartoma in a dog

Necroptosis-Related Prognostic Model for Pancreatic Carcinoma Reveals Its Invasion and Metastasis Potential through Hybrid EMT and Immune Escape

Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration

Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

A Comparative Evaluation of Concentrated Growth Factor and Platelet-rich Fibrin on the Proliferation, Migration, and Differentiation of Human Stem Cells of the Apical Papilla

MicroRNA-421 mediates immunosensitivity in late-stage human liver cancer

Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen

Expression of nerve growth factor in skin tissues and its effect on the proliferation of outer root sheath cells in cashmere goats

Establishment of a corneal epithelial cell line spontaneously derived from human limbal cells

Adult islets cultured in collagen gel transdifferentiate into duct-like cells

Review

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