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Product Info Summary
| SKU: | A30471 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-K6PP Antibody
SKU/Catalog Number
A30471
Size
100ul
Form
Liquid
Description
Boster Bio Anti-K6PP Antibody (Catalog# A30471). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K6PP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30471)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human K6PP. Immunogen sequence location: 341-390
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30471 is reactive to PFKP in Human, Monkey, Mouse, Rat
Applications
A30471 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
85.596kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from A549 and COS7 cells, using K6PP Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For PFKP (Source: Uniprot.org, NCBI)
Gene Name
PFKP
Full Name
ATP-dependent 6-phosphofructokinase, platelet type
Weight
85.596kDa
Superfamily
phosphofructokinase type A (PFKA) family
Alternative Names
6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; EC 2.7.1; EC 2.7.1.11; PFK-CFLJ40226; PFKFplatelet type; Phosphofructokinase 1; phosphofructokinase, platelet; phosphohexokinase PFKP ATP-PFK, PFK-C, PFK-P, PFKF phosphofructokinase, platelet ATP-dependent 6-phosphofructokinase, platelet type|6-phosphofructokinase type C|6-phosphofructokinase, platelet type|phosphofructo-1-kinase isozyme C|phosphofructokinase 1|phosphohexokinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKP, check out the PFKP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K6PP Antibody (A30471)
Hello CJ!
No publications found for A30471
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-K6PP Antibody
Question
Does A30471 anti-K6PP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Taylor
Verified customer
Asked: 2018-10-23
Answer
You can see on the product datasheet, A30471 anti-K6PP antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-23
Question
Can you help my question with product A30471, anti-K6PP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30471 anti-K6PP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-05
Question
We are currently using anti-K6PP antibody A30471 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The anti-K6PP antibody (A30471) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
