Anti-Kallikrein 10/KLK10 Antibody

Product Name

Anti-Kallikrein 10/KLK10 Antibody

View all Kallikrein 10 Antibodies

SKU/Catalog Number

PA1821

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Kallikrein 10/KLK10 Antibody catalog # PA1821. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Kallikrein 10/KLK10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1821)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1821 is reactive to KLK10 in Human, Rat

Applications

PA1821 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

30.17kDa

Background of Kallikrein 10

KLK10 (kallikrein-related peptidase 10) also known as kallikrein 10, PRSSL1 or NES1, belongs to the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers.KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adult and fetal tissues examined. The KLK10 gene contains 6 exons, the first of which is noncoding. By fluorescence in situ hybridization, Polikoff et al. (1997) mapped the PRSSL1 gene to 19q13.3. By genomic sequence analysis, Gan et al. (2000) mapped the KLK10 gene within the KLK gene cluster on chromosome 19q13. This cluster includes 13 KLK genes and 5 pseudogenes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Anti-Kallikrein 10 antibody, PA1821, Western blotting
All lanes: Anti Kallikrein 10 (PA1821) at 0.5ug/ml
Lane 1: Rat Ovary Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Liver Tissue Lysate at 50ug
Lane 5: 22RV Whole Cell Lysate at 40ug
Lane 6: SROV Whole Cell Lysate at 40ug
Lane 7: HELA Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD

Gene/Protein Information For KLK10 (Source: Uniprot.org, NCBI)

Alternative Names

breast normal epithelial cell associated serine protease; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.74; Kallikrein 10; kallikrein-related peptidase 10; KLK10; NES1; NES1kallikrein-10; Normal epithelial cell-specific 1; Protease serine-like 1; PRSSL1protease, serine-like, 1 KLK10 NES1, PRSSL1 kallikrein related peptidase 10 kallikrein-10|breast normal epithelial cell associated serine protease|kallikrein 10 protein 1|kallikrein 10 protein 12|kallikrein 10 protein 13|kallikrein 10 protein 2|kallikrein 10 protein 3|kallikrein 10 protein 4|kallikrein 10 protein 5|kallikrein 10 protein 7|kallikrein 10 protein 8|kallikrein 10 protein 9|normal epithelial cell-specific 1|protease serine-like 1

For more info on KLK10, check out the KLK10 Infographic

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In this infographic, you will see the following information for KLK10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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