Anti-KANK2/Ankrd25 Antibody

KANK2 antibody

Boster Bio Anti-KANK2/Ankrd25 Antibody (Catalog # A07999). Tested in IHC, IF applications. This antibody reacts with Human.

Product Info Summary

SKU: A07999
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC

Product Name

Anti-KANK2/Ankrd25 Antibody

View all KANK2 Antibodies

SKU/Catalog Number

A07999

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-KANK2/Ankrd25 Antibody (Catalog # A07999). Tested in IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KANK2/Ankrd25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07999)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human KANK2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07999 is reactive to KANK2 in Human

Applications

A07999 is guaranteed for IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

91.174kDa

Background of KANK2

Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation.

Nagase T., DNA Res. 7:143-150(2000).
Ota T., Nat. Genet. 36:40-45(2004).
Olsen J.V., Cell 127:635-648(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For KANK2 (Source: Uniprot.org, NCBI)

Gene Name

KANK2

Full Name

KN motif and ankyrin repeat domain-containing protein 2

Weight

91.174kDa

Alternative Names

ANKRD25; ankyrin repeat domain 25; Ankyrin repeat domain-containing protein 25; DKFZp434N161; FLJ20004; KIAA1518SRC1-interacting protein; kidney ankyrin repeat-containing protein 2; KN motif and ankyrin repeat domain-containing protein 2; KN motif and ankyrin repeat domains 2; Matrix-remodeling-associated protein 3; matrix-remodelling associated 3; MGC119707; MXRA3; SIP; SRC-1 interacting protein; SRC-1-interacting protein KANK2 ANKRD25, MXRA3, NPHS16, PPKWH, SIP KN motif and ankyrin repeat domains 2 KN motif and ankyrin repeat domain-containing protein 2|SRC-interacting protein|SRC1-interacting protein|ankyrin repeat domain-containing protein 25|kidney ankyrin repeat-containing protein 2|matrix-remodeling-associated protein 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KANK2, check out the KANK2 Infographic

KANK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KANK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-KANK2/Ankrd25 Antibody

Question

We need to test anti-KANK2/Ankrd25 antibody A07999 on human cervix lung for research purposes, then I may be interested in using anti-KANK2/Ankrd25 antibody A07999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

The products we sell, including anti-KANK2/Ankrd25 antibody A07999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-27

Question

Is there a BSA free version of anti-KANK2/Ankrd25 antibody A07999 available?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KANK2/Ankrd25 antibody A07999 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix lung using anti-KANK2/Ankrd25 antibody A07999. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-15

Question

We are currently using anti-KANK2/Ankrd25 antibody A07999 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

The anti-KANK2/Ankrd25 antibody (A07999) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-11

Question

Is a blocking peptide available for product anti-KANK2/Ankrd25 antibody (A07999)?

G. Thomas

Verified customer

Asked: 2018-10-11

Answer

We do provide the blocking peptide for product anti-KANK2/Ankrd25 antibody (A07999). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-10-11

Question

Would anti-KANK2/Ankrd25 antibody A07999 work on horse IHC with liver?

Verified Customer

Verified customer

Asked: 2017-09-12

Answer

Our lab technicians have not tested anti-KANK2/Ankrd25 antibody A07999 on horse. You can run a BLAST between horse and the immunogen sequence of anti-KANK2/Ankrd25 antibody A07999 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-12

Question

Would A07999 anti-KANK2/Ankrd25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-08-29

Answer

You can see on the product datasheet, A07999 anti-KANK2/Ankrd25 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-08-29

Order DetailsPrice
A07999-100uL

100uL

$360

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