Click image to see more details
Product Info Summary
| SKU: | A02764 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ARHGEF7/Beta Pix Antibody
SKU/Catalog Number
A02764
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ARHGEF7/Beta Pix Antibody (Catalog# A02764). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARHGEF7/Beta Pix Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02764)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ARHGEF7. Immunogen sequence location: 311 - 360
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02764 is reactive to ARHGEF7 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
90.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02764 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:5000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from rat muscle and rat heart cells, using ARHGEF7 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ARHGEF7/Beta Pix Antibody (A02764)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-ARHGEF7/Beta Pix Antibody
Question
Does anti-ARHGEF7/Beta Pix antibody A02764 work on horse WB with neuroblastoma?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Our lab technicians have not validated anti-ARHGEF7/Beta Pix antibody A02764 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ARHGEF7/Beta Pix antibody A02764 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse neuroblastoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-ARHGEF7/Beta Pix antibody A02764. Let me know if you need anything else.
D. Jones
Verified customer
Asked: 2015-03-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-13
Question
We are currently using anti-ARHGEF7/Beta Pix antibody A02764 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
T. Krishna
Verified customer
Asked: 2013-11-11
Answer
The anti-ARHGEF7/Beta Pix antibody (A02764) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-11
