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- Table of Contents
EP300 antibody (A00117) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of 293T cells using anti-KAT3B/P300/EP300 antibody (A00117).
Overlay histogram showing 293T cells stained with A00117 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT3B/P300/EP300 Antibody (A00117, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing 293T cells stained with A00117 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT3B/P300/EP300 Antibody (A00117, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for EP300? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the EP300 Western Blot GuideBundle Save
Anti-KAT3B/p300/EP300 Antibody Picoband®
EP300 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
EP300 antibody was positively detected by WB in:
human 293T whole cell, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
EP300 antibody was positively detected by IHC in:
differentiated adenocarcinoma of the human rectum tissue, human classic Hodgkin's lymphoma tissue, human gastric signet ring cell carcinoma tissue, human placenta tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
EP300 antibody was positively detected by ICC/IF in:
SiHa cell
EP300 antibody was positively detected by FC in:
293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-KAT3B/p300/EP300 Antibody Picoband®
View all EP300 Antibodies
View all EP300 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KAT3B/p300/EP300, which shares 76.5% amino acid (aa) sequence identity with mouse EP300.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
264-300 kDa
Calculated Molecular Weight
264.2 kDa
Background of EP300
E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Cite This Product
Anti-KAT3B/p300/EP300 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00117)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Rabbit
82%
Moderate Homology
Dog
82%
Moderate Homology
Mouse
76%
Moderate Homology
Cow
76%
Moderate Homology
Rat
71%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for EP300 antibody
Immunohistochemistry (IHC) protocol for EP300 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for EP300 antibody
Flow Cytometry (FC) protocol for EP300 antibody
Publications
Application:
Species:
Amorphophallus konjac via Gut Microbiota-FXR/FGF15 Axis Changed Bile Acid Metabolism in Hyperlipidemia Mice
Published: 2025-08-06
Journal: JOURNAL OF FOOD BIOCHEMISTRY
Application: IF
Impact Factor: 4.20
Species: Mouse
Oncostatin M/Oncostatin M Receptor Signal Induces Radiation-Induced Heart Fibrosis by Regulating SMAD4 in Fibroblast
Published: 2023-08-23
Journal: INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
Application: IHC
Impact Factor: 7.00
Species: Mouse
PMID: 37610394
Effects of PM2.5 exposure in utero on heart injury, histone acetylation and GATA4 expression in offspring mice
Published: 2020-05-19
Journal: CHEMOSPHERE
Application: WB
Impact Factor: 5.78
Species: Mouse
PMID: 32454355
The key role of macrophage depolarization in the treatment of COPD with ergosterol both in vitro and in vivo
Published: 2019-12-23
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB,IHC
Impact Factor: 3.36
Species: Rat
PMID: 31874368
Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice
Published: 2011-04-21
Journal: Chinese Journal of Integrative Medicine
Application: IHC
Impact Factor: 0.58
Species: Mouse
PMID: 21509671
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Product Promise
We promise Anti-KAT3B/p300/EP300 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00117
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026