ko-validated

EP300 antibody (PB9178) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 2 Publications
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Anti-KAT3B/p300/EP300 Antibody Picoband®

EP300 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitIHC, WB

SKU: PB9178

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

2 in stock. Ships Next Business Day.

Quantity: 1

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Tested Applications

EP300 antibody was positively detected by WB in:

human HeLa cells, human Caco-2 cells, rat C6 cells, mouse NIH3T3 cells, human Hela- WT whole cell lysates, human Hela-EP300 KO whole cell lysates

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

EP300 antibody was positively detected by IHC in:

mouse intestine tissue, rat intestine tissue, human oesophagus squama cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-KAT3B/p300/EP300 Antibody Picoband®
View all EP300 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
300 kDa
Calculated Molecular Weight

264.2 kDa

Background of EP300
E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Cite This Product
Anti-KAT3B/p300/EP300 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9178)

Protocols

Western Blot protocol for EP300 antibody
Immunohistochemistry (IHC) protocol for EP300 antibody

Publications

Application:
Species:
EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT
Published: 2026-08-26
Journal: CLINICAL AND EXPERIMENTAL MEDICINE
Application: IHC,WB,IF
Impact Factor: 4.50
Species: Human,Mouse
MAFG-AS1/MAFG positive feedback loop contributes to cisplatin resistance in bladder urothelial carcinoma through antagonistic ferroptosis
Published: 2021-01-26
Journal: Science Bulletin
Application: WB
Impact Factor: 11.78
Species: Human
PMID: 36654385

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Product Promise

We promise Anti-KAT3B/p300/EP300 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PB9178
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 9, 2026