Anti-KAT3B/p300/EP300 Antibody Picoband™

KAT3B/p300 antibody

Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband™ catalog # PB9178. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9178
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-KAT3B/p300/EP300 Antibody Picoband™

View all KAT3B/p300 Antibodies

SKU/Catalog Number

PB9178

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KAT3B/p300/EP300 Antibody Picoband™ catalog # PB9178. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KAT3B/p300/EP300 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9178)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9178 is reactive to EP300 in Human, Mouse, Rat

Applications

PB9178 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

300 kDa

Calculated molecular weight

264.161kDa

Background of KAT3B/p300

E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EP300 (Source: Uniprot.org, NCBI)

Gene Name

EP300

Full Name

Histone acetyltransferase p300

Weight

264.161kDa

Alternative Names

E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; EC 2.3.1; EC 2.3.1.48; EP300; histone acetyltransferase p300; KAT3B; p300 HAT; p300; RSTS2 EP300 KAT3B, MKHK2, RSTS2, p300 E1A binding protein p300 histone acetyltransferase p300|E1A-associated protein p300|E1A-binding protein, 300kD|histone butyryltransferase p300|histone crotonyltransferase p300|p300 HAT|protein 2-hydroxyisobutyryltransferase p300|protein propionyltransferase p300

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EP300, check out the EP300 Infographic

EP300 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EP300: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9178 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

MAFG-AS1/MAFG positive feedback loop contributes to cisplatin resistance in bladder urothelial carcinoma through antagonistic ferroptosis

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12 Customer Q&As for Anti-KAT3B/p300/EP300 Antibody Picoband™

Question

We were content with the WB result of your anti-KAT3B/p300/EP300 antibody. However we have seen positive staining in cervix carcinoma erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is EP300 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

From literature, cervix carcinoma erythroleukemia does express EP300. Generally EP300 expresses in cytoplasm. Regarding which tissues have EP300 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-10-09

Question

Does anti-KAT3B/p300/EP300 antibody PB9178 work on goat WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Our lab technicians have not tested anti-KAT3B/p300/EP300 antibody PB9178 on goat. You can run a BLAST between goat and the immunogen sequence of anti-KAT3B/p300/EP300 antibody PB9178 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-09

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-KAT3B/p300/EP300 antibody PB9178. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-13

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-KAT3B/p300/EP300 antibody PB9178. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-02-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-27

Question

We have observed staining in mouse cervix carcinoma erythroleukemia. What should we do? Is anti-KAT3B/p300/EP300 antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

Based on literature cervix carcinoma erythroleukemia does express EP300. Based on Uniprot.org, EP300 is expressed in kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have EP300 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-12-05

Question

I was wanting to use your anti-KAT3B/p300/EP300 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2018-10-04

Answer

As indicated on the product datasheet, PB9178 anti-KAT3B/p300/EP300 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-04

Question

We are currently using anti-KAT3B/p300/EP300 antibody PB9178 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-17

Answer

The anti-KAT3B/p300/EP300 antibody (PB9178) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-17

Question

We bought anti-KAT3B/p300/EP300 antibody for WB on leukemic t-cell in the past. I am using human, and I plan to use the antibody for IHC next. I am interested in examining leukemic t-cell as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2017-10-30

Answer

I have checked the website and datasheets of our anti-KAT3B/p300/EP300 antibody and it seems that PB9178 has been tested on human in both WB and IHC. Thus PB9178 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-10-30

Question

I am interested in using your anti-KAT3B/p300/EP300 antibody for histone h4 acetylation studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-09-01

Answer

Thanks for your inquiry. This PB9178 anti-KAT3B/p300/EP300 antibody is validated on rat intestine tissue, mouse intestine tissue, nih3t3 whole cell lysates, squama cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-09-01

Question

Is this PB9178 anti-KAT3B/p300/EP300 antibody reactive to the isotypes of EP300?

R. Li

Verified customer

Asked: 2015-06-25

Answer

The immunogen of PB9178 anti-KAT3B/p300/EP300 antibody is E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-06-25

Question

Does anti-KAT3B/p300/EP300 antibody PB9178 work for WB with cervix carcinoma?

L. Baker

Verified customer

Asked: 2014-11-27

Answer

According to the expression profile of cervix carcinoma, EP300 is highly expressed in cervix carcinoma. So, it is likely that anti-KAT3B/p300/EP300 antibody PB9178 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2014-11-27

Question

We are interested in to test anti-KAT3B/p300/EP300 antibody PB9178 on rat cervix carcinoma for research purposes, then I may be interested in using anti-KAT3B/p300/EP300 antibody PB9178 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Parker

Verified customer

Asked: 2013-04-02

Answer

The products we sell, including anti-KAT3B/p300/EP300 antibody PB9178, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-04-02

Order DetailsPrice
PB9178

100μg

$370
PB9178-10ug

10μg sample (liquid)

$99
PB9178-Biotin

100 μg Biotin conjugated

$570
PB9178-Cy3

100 μg Cy3 conjugated

$570
PB9178-Dylight488

100 μg Dylight488 conjugated

$570
PB9178-Dylight550

100 μg Dylight550 conjugated

$570
PB9178-Dylight594

100 μg Dylight594 conjugated

$570
PB9178-FITC

100 μg FITC conjugated

$570
PB9178-HRP

100 μg HRP conjugated

$570
PB9178-APC

100 μg APC conjugated

$670
PB9178-PE

100 μg PE conjugated

$670
PB9178-carrier-free

Carrier Free

$370

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