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Product Info Summary
| SKU: | M02757-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KAT8 / MYST1 / MOF Rabbit Monoclonal Antibody
SKU/Catalog Number
M02757-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-KAT8 / MYST1 / MOF Rabbit Monoclonal Antibody catalog # M02757-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT8 / MYST1 / MOF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02757-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
19K10
Isotype
IgG
Immunogen
A synthesized peptide derived from human KAT8 / MYST1 / MOF
Reactive Species
M02757-1 is reactive to KAT8 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02757-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:100
FC 1:100
Positive Control
WB: HeLa cell
IHC: human colorectal adenocarcinoma tissue, human breast cancer tissue, human lung squamous cell carcinoma tissue, human placenta tissue, human thyroid cancer tissue
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Western blot analysis of KAT8 / MYST1 / MOF expression in HeLa cell lysate.
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IHC analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1).
KAT8 / MYST1 / MOF was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1).
KAT8 / MYST1 / MOF was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1).
KAT8 / MYST1 / MOF was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1).
KAT8 / MYST1 / MOF was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1).
KAT8 / MYST1 / MOF was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IF analysis of KAT8 / MYST1 / MOF using anti-KAT8 / MYST1 / MOF antibody (M02757-1) and anti-Beta Tubulin antibody (M01857-3).
KAT8 / MYST1 / MOF was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:100 with rabbit anti-KAT8 / MYST1 / MOF Antibody (M02757-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Dylight550-conjugated Anti- mouse IgG Secondary Antibody (red)(Catalog#BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-KAT8 / MYST1 / MOF Rabbit Monoclonal Antibody (M02757-1)
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