Product Info Summary
| SKU: | A04502-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-KCNK1 Antibody
SKU/Catalog Number
A04502-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KCNK1 Antibody (Catalog # A04502-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KCNK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-KCNK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04502-1)
Host
Rabbit
Contents
KCNK1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KCNK1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KCNK1. The immunogen is located within amino acids 220 - 270 of KCNK1.
Cross-reactivity
KCNK1 antibody is human, mouse and rat reactive. KCNK1 antibody is predicted to not cross-react with other KCNK proteins.
Reactive Species
A04502-1 is reactive to KCNK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.1 kDa
Background of KCNK1
The potassium channel, subfamily K, member 1 (KCNK1), is member of the superfamily of potassium channel proteins containing two pore-forming P domains (1,2). KCNK1 is a ubiquitous human weakly inward rectifying K+ channel that is widely expressed in human tissues, and particularly abundant in brain and heart (1). Both KCNK1 and the related protein KCNK2 contribute significantly to the passive conductance of hippocampal astrocytes (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04502-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KCNK1 antibody can be used for detection of KCNK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KCNK1 in 3T3 cell lysate with KCNK1 antibody at 1 μg/ml.
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Immunohistochemistry of KCNK1 in mouse brain tissue with KCNK1 antibody at 5 μg/ml.
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Immunofluorescence of KCNK1 in mouse brain tissue with KCNK1 antibody at 20 μg/ml.
Specific Publications For Anti-KCNK1 Antibody (A04502-1)
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