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Product Info Summary
| SKU: | A14172-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-KCNK12 Antibody
SKU/Catalog Number
A14172-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KCNK12 Antibody (Catalog # A14172-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KCNK12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KCNK12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14172-1)
Host
Rabbit
Contents
KCNK12 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12. The immunogen is located within amino acids 320 - 370 of KCNK12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
KCNK12 antibody is predicted to not cross-react with other KCNK protein family members.
Reactive Species
A14172-1 is reactive to KCNK12 in Human, Mouse, Rat
Applications
A14172-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
46.889kDa
Background of KCNK12
KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle. KCNK12 is closely related to KCNK13 (58% identity at the amino acid level), but could not yet been functionally expressed in vitro and may require other proteins to become active.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KCNK12 in rat brain tissue lysate with KCNK12 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of KCNK12 in mouse brain tissue with KCNK12 antibody at 5 μg/mL.
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Immunofluorescence of KCNK12 in mouse brain tissue with KCNK12 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For KCNK12 (Source: Uniprot.org, NCBI)
Gene Name
KCNK12
Full Name
Potassium channel subfamily K member 12
Weight
46.889kDa
Superfamily
two pore domain potassium channel (TC 1.A.1.8) family
Alternative Names
potassium channel subfamily K member 12; potassium channel, subfamily K, member 12; tandem pore domain potassium channel THIK-2; THIK2; THIK-2; THIK-2THIK2Tandem pore domain halothane-inhibited potassium channel 2 KCNK12 K2p12.1, THIK-2, THIK2 potassium two pore domain channel subfamily K member 12 potassium channel subfamily K member 12|potassium channel, subfamily K, member 12|potassium channel, two pore domain subfamily K, member 12|tandem pore domain halothane-inhibited potassium channel 2|tandem pore domain potassium channel THIK-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KCNK12, check out the KCNK12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KCNK12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KCNK12 Antibody (A14172-1)
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No publications found for A14172-1
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