|Product Name||Anti-KCNN4 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Intermediate conductance calcium-activated potassium channel protein 4(KCNN4) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-KCNN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1047-1)|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human KCNN4(14-29aa RRKRLLEQEKSLAGWA), different from the related mouse and rat sequences by two amino acids.|
Assay Dilutions Overview
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-KCNN antibody, PA1047-1, Western blotting
All lanes: Anti KCNN(PA1047-1) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Intermediate conductance calcium-activated potassium channel protein 4|
|Tissue Specificity||Widely expressed in non-excitable tissues.|
|Alternative Names||Intermediate conductance calcium-activated potassium channel protein 4;SK4;SKCa 4;SKCa4;IKCa1;IK1;KCa3.1;KCa4;Putative Gardos channel;KCNN4;IK1, IKCA1, KCA4, SK4;|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Molecular Weight||47696 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. .|
|Background||Intermediate conductance calcium-activated potassium channel protein 1(KCNN4, Kca3.1) is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. KCNN4 may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.|
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,