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| SKU | PA1047-1 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Rat |
| Clonality | Polyclonal |
| Host | Rabbit |
| Ig Isotype | N/A |
| Applications | WB |
Overview
| Product Name | Anti-KCNN4 Antibody |
|---|---|
| SKU/Catalog Number | PA1047-1 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Rabbit IgG polyclonal antibody for Intermediate conductance calcium-activated potassium channel protein 4(KCNN4) detection. Tested with WB in Human;Rat. |
| Cite This Product | Anti-KCNN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1047-1) |
| Host | Rabbit |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human KCNN4(14-29aa RRKRLLEQEKSLAGWA), different from the related mouse and rat sequences by two amino acids. |
| Reactivity | Human, Rat |
Assay Details
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human, Rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.Images And Assay Conditions
Anti-KCNN antibody, PA1047-1, Western blotting
All lanes: Anti KCNN(PA1047-1) at 0.5ug/ml
WB: HUT Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | O15554 |
|---|---|
| Gene Name | KCNN4 |
| Protein Name | Intermediate conductance calcium-activated potassium channel protein 4 |
| Tissue Specificity | Widely expressed in non-excitable tissues. |
| Alternative Names | Intermediate conductance calcium-activated potassium channel protein 4;SK4;SKCa 4;SKCa4;IKCa1;IK1;KCa3.1;KCa4;Putative Gardos channel;KCNN4;IK1, IKCA1, KCA4, SK4; |
| Subcellular Localization | Membrane; Multi-pass membrane protein. |
| Molecular Weight | 47696 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. . |
|---|---|
| Background | Intermediate conductance calcium-activated potassium channel protein 1(KCNN4, Kca3.1) is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. KCNN4 may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. |
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Customer Q&As
Q: Do you offer BSA-free antibodies?
Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.