Product Info Summary
| SKU: | A08651 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-KCTD15 Antibody
SKU/Catalog Number
A08651
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KCTD15 Antibody (Catalog # A08651). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KCTD15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KCTD15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08651)
Host
Rabbit
Contents
KCTD15 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KCTD15 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human KCTD15. The immunogen is located within the last 50 amino acids of KCTD15.
Reactive Species
A08651 is reactive to KCTD15 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.9 kDa
Background of KCTD15
Childhood and adult obesity in the United States and to a lesser extent the rest of the world has increased dramatically over the past decade. Both environmental and genetic factors are involved in the onset and progression of weight gain. Recently, the potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Further studies on single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels. At least two isoforms of KCTD15 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08651 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KCTD15 antibody can be used for detection of KCTD15 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KCTD15 in HeLa cell lysate with KCTD15 antibody at 1μg/ml in (A) the absence and (B) the presence of blocking buffer.
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Immunohistochemistry of KCTD15 in human spleen tissue with KCTD15 antibody at 2.5 μg/mL.
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Immunofluorescence of KCTD15 in human spleen tissue with KCTD15 antibody at 20 μg/mL.
Specific Publications For Anti-KCTD15 Antibody (A08651)
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