Anti-KDEL Receptor Monoclonal Antibody

Product Name

Anti-KDEL Receptor Monoclonal Antibody

View all KDELR1 Antibodies

SKU/Catalog Number

M07617-2

Size

100ug

Form

liquid

Description

Boster Bio Anti-KDEL Receptor Monoclonal Antibody catalog # M07617-2. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Dog, Fruit fly, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Frog.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KDEL Receptor Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07617-2)

Host

Mouse

Contents

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

KR-10

Isotype

IgG1

Immunogen

A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~25kDa.

Reactive Species

M07617-2 is reactive to KDELR1 in Bovine, Chicken, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus

Applications

M07617-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

24.542kDa

Background of KDELR1

The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Click image to see more details

Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse AntiKDEL Receptor Monoclonal Antibody, Clone KR-10 (M07617-2). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (M07617-2) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Click image to see more details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (M07617-2) . Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (M07617-2) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red) . Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.

Gene/Protein Information For KDELR1 (Source: Uniprot.org, NCBI)

Alternative Names

ER lumen protein retaining receptor 1; ERD2; ERD2.1KDEL receptor 1; HDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; PM23; Putative MAPK-activating protein PM23 KDELR1 ERD2, ERD2.1, HDEL, PM23 KDEL endoplasmic reticulum protein retention receptor 1 ER lumen protein-retaining receptor 1|KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1|KDEL receptor 1|putative MAPK-activating protein PM23

For more info on KDELR1, check out the KDELR1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KDELR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the KDELR1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis