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Product Info Summary
| SKU: | A08071 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-KDM1B Antibody
SKU/Catalog Number
A08071
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KDM1B Antibody (Catalog # A08071). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
KDM1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-KDM1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08071)
Host
Rabbit
Contents
KDM1B antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
KDM1B antibody was raised against a 17 amino acid peptide near the amino terminus of human KDM1B. The immunogen is located within amino acids 80 - 130 of KDM1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.
Reactive Species
A08071 is reactive to Kdm1b in Human, Mouse
Applications
A08071 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
92.633kDa
Background of AOF1
Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KDM1B antibody can be used for detection of KDM1B by Western blot at 0.5 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KDM1B in K562 cell lysate with KDM1B antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide
Protein Target Info & Infographic
Gene/Protein Information For Kdm1b (Source: Uniprot.org, NCBI)
Gene Name
Kdm1b
Full Name
Lysine-specific histone demethylase 1B
Weight
92.633kDa
Superfamily
flavin monoamine oxidase family
Alternative Names
AOF1; bA204B7.3; C6orf193; dJ298J15.2; DKFZp686I0412; flavin containing 1; flavin-containing amine oxidase domain-containing protein 1; FLJ33898; FLJ34109; FLJ43328; LSD2; lysine (K)-specific demethylase 1B; lysine-specific histone demethylase 1B; lysine-specific histone demethylase 2 Kdm1b|4632428N09Rik, AI482520, Ao, Aof1|lysine (K)-specific demethylase 1B|lysine-specific histone demethylase 1B|amine oxidase, flavin containing 1|flavin-containing amine oxidase domain-containing protein 1|lysine-specific histone demethylase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Kdm1b, check out the Kdm1b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Kdm1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KDM1B Antibody (A08071)
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No publications found for A08071
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