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- Table of Contents
KEAP1 antibody (A00514) performs well in ELISA, IF, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescence Validation of KEAP1 in Human Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling KEAP1 with A00514 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling KEAP1 with A00514 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-KEAP1 Antibody
KEAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-KEAP1 Antibody
View all KEAP1 Antibodies
View all KEAP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
KEAP1 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
KEAP1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.
Cross-Reactivity
At least two isoforms of KEAP1 are known to exist.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
69.7 kDa
Background of KEAP1
KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.
Cite This Product
Anti-KEAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514)
Publications
Application:
Species:
Li D,Bai X,Jiang Y,Cheng Y.Butyrate alleviates PTZ-induced mitochondrial dysfunction, oxidative stress and neuron apoptosis in mice via Keap1/Nrf2/HO-1 pathway.Brain Res Bull.2020 Dec 28;168:25-35.doi:10.1016/j.brainresbull.2020.12.009.Epub ahead of print
Species: Mouse
PMID: 33359640
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Product Promise
We promise Anti-KEAP1 Antibody performs excellently in relevant applications for ELISA, IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00514
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026