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Product Info Summary
| SKU: | A00514 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-KEAP1 Antibody
SKU/Catalog Number
A00514
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KEAP1 Antibody (Catalog # A00514). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-KEAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514)
Host
Rabbit
Contents
KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.
Cross-reactivity
At least two isoforms of KEAP1 are known to exist.
Reactive Species
A00514 is reactive to KEAP1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
69.7 kDa
Background of KEAP1
KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00514 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation of KEAP1 in Human Lung
Loading: 15 μg of lysates per lane.
Antibodies: KEAP1 A00514 (A: 1 μg/mL; B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: KEAP1 A00514 (2 μg/mL), KEAP1 22-002 (2 μg/mL, and GAPHDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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KO Validation in HeLa cells
Loading: 10 μg of HeLa WT cell lysates or KEAP1 KO cell lysates. Antibodies: KEAP1 A00514 (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of KEAP1 in Human Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling KEAP1 with A00514 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-KEAP1 Antibody (A00514)
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