Anti-KEAP1 Antibody

Product Name

Anti-KEAP1 Antibody

View all KEAP1 Antibodies

SKU/Catalog Number

A00514

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-KEAP1 Antibody (Catalog # A00514). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-KEAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00514)

Host

Rabbit

Contents

KEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of KEAP1 are known to exist.

Reactive Species

A00514 is reactive to KEAP1 in Human, Mouse, Rat

Applications

A00514 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

69.666kDa

Background of KEAP1

KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κB activity by targeting IKKβ degradation. Mutation of the KEAP1 gene is found in lung cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation of KEAP1 in Human Lung
Loading: 15 μg of lysates per lane.Antibodies: KEAP1 A00514 (A: 1 μg/mL; B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KEAP1 A00514 (2 μg/mL), KEAP1 (2 μg/mL, and GAPHDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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KO Validation in HeLa cells
Loading: 10 μg of HeLa WT cell lysates or KEAP1 KO cell lysates. Antibodies: KEAP1 A00514 (2 μg/mL) and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

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Immunofluorescence Validation of KEAP1 in Human Lung
Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling KEAP1 with A00514 at 20 μg/mL

Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)

Alternative Names

INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887 KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19

For more info on KEAP1, check out the KEAP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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