Anti-KEPI/PPP1R14C Antibody Picoband™

Product Name

Anti-KEPI/PPP1R14C Antibody Picoband™

View all Ppp1r14c Antibodies

SKU/Catalog Number

A10973-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-KEPI/PPP1R14C Antibody Picoband™ catalog # A10973-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KEPI/PPP1R14C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10973-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human KEPI/PPP1R14C recombinant protein (Position: H67-V165).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10973-2 is reactive to Ppp1r14c in Human, Mouse, Rat

Applications

A10973-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

17.754kDa

Background of Ppp1r14c

Protein phosphatase 1 regulatory subunit 14C is an enzyme that in humans is encoded by the PPP1R14C gene. The degree of protein phosphorylation is regulated by a balance of protein kinase and phosphatase activities. Protein phosphatase-1 (PP1; see MIM 176875) is a signal-transducing phosphatase that influences neuronal activity, protein synthesis, metabolism, muscle contraction, and cell division. PPP1R14C is an inhibitor of PP1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (A10973-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat H9C2(2-1) whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (Catalog # A10973-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-KEPI/PPP1R14C antibody (A10973-2).
Overlay histogram showing 293T cells stained with A10973-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEPI/PPP1R14C Antibody (A10973-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Ppp1r14c (Source: Uniprot.org, NCBI)

Alternative Names

Protein phosphatase 1 regulatory subunit 14C Ppp1r14c|6330514J04Rik, KE, KEPI|protein phosphatase 1, regulatory inhibitor subunit 14C|protein phosphatase 1 regulatory subunit 14C|PKC-potentiated PP1 inhibitory protein|kinase-enhanced PP1 inhibitor

For more info on Ppp1r14c, check out the Ppp1r14c Infographic

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In this infographic, you will see the following information for Ppp1r14c: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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