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Product Info Summary
| SKU: | A07712-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Keratocan/KTN/Kera Antibody Picoband®
SKU/Catalog Number
A07712-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Keratocan/KTN/Kera Antibody Picoband® catalog # A07712-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Keratocan/KTN/Kera Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07712-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Keratocan/KTN/Kera recombinant protein (Position: R73-K115).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07712-1 is reactive to Kera in Mouse, Rat
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.4 kDa
Background of Kera
Keratocan (KTN) also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07712-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat eye tissue, mouse eye tissue
Validation Images & Assay Conditions
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Western blot analysis of Keratocan/KTN/Kera using anti-Keratocan/KTN/Kera antibody (A07712-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat eye tissue lysates,
Lane 2: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan/KTN/Kera antigen affinity purified polyclonal antibody (Catalog # A07712-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan/KTN/Kera at approximately 41 kDa. The expected band size for Keratocan/KTN/Kera is at 41 kDa.
Specific Publications For Anti-Keratocan/KTN/Kera Antibody Picoband® (A07712-1)
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