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Product Info Summary
| SKU: | A16774 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-KHDC1 Antibody
SKU/Catalog Number
A16774
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-KHDC1 Antibody (Catalog # A16774). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
KHDC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-KHDC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16774)
Host
Rabbit
Contents
KHDC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KHDC1 antibody was raised against a 15 amino acid peptide near the amino terminus of human KHDC1. The immunogen is located within amino acids 30 - 80 of KHDC1.
Cross-reactivity
KHDC1 antibody is human, mouse and rat reactive. At least three isoforms of KHDC1 are known to exist; this antibody will only detect the longest isoform.
Reactive Species
A16774 is reactive to KHDC1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.2 kDa
Background of KHDC1
KHDC1 family members are K-homology (KH) domain-containing RNA binding proteins that are involved in various aspects of RNA metabolism, ranging from transcription to RNA splicing, transportation, translation, and stability (1). KHDC1 is highly expressed in oocytes and interacts with cytoplasmic polyadenylation element-binding protein 1 (CPEB1), a key translational regulator controlling the polyA length of mRNAs (2,3). KHDC1 functions as a global translational repressor and induces apoptosis through an ER-dependent signaling pathway and that the C-terminal putative trans-membrane motif (TMM) is critical for its activities (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16774 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KHDC1 antibody can be used for detection of KHDC1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KHDC1 in rat liver tissue lysate with KHDC1 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of KHDC1 in human liver tissue with KHDC1 antibody at 5 μg/mL.
Specific Publications For Anti-KHDC1 Antibody (A16774)
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