Anti-KIF5 Antibody

Product Name

Anti-KIF5 Antibody

View all KIF5A Antibodies

SKU/Catalog Number

A03635

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-KIF5 Antibody (Catalog # A03635). Tested in ELISA, WB, IHC, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KIF5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-KIF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03635)

Host

Rabbit

Contents

KIF5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5. The immunogen is located within amino acids 880 - 930 of KIF5.

Cross-reactivity

At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.

Reactive Species

A03635 is reactive to KIF5A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

117.4 kDa

Background of KIF5A

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03635 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information KIF5A

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