Anti-KIF5 Antibody

Product Name

Anti-KIF5 Antibody

View all Kinesin 5A Antibodies

SKU/Catalog Number

A03635

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-KIF5 Antibody (Catalog # A03635). Tested in ELISA, WB, IHC, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KIF5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-KIF5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03635)

Host

Rabbit

Contents

KIF5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5. The immunogen is located within amino acids 880 - 930 of KIF5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.

Reactive Species

A03635 is reactive to KIF5A in Human, Mouse, Rat

Applications

A03635 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

117.378kDa

Background of Kinesin 5A

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL and Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 μg/mL.

Click image to see more details

Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 μg/mL.

Click image to see more details

Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 μg/mL.

Click image to see more details

Immunohistochemistry of KIF5 in mouse brain tissue with KIF5 antibody at 5 μg/ml.

Click image to see more details

Immunofluorescence of KIF5 in mouse brain tissue with KIF5 antibody at 20 μg/mL.

Red: KIF5 Antibody (A03635)
Blue: DAPI staining

Gene/Protein Information For KIF5A (Source: Uniprot.org, NCBI)

Alternative Names

D12S1889; KIF5A variant protein; kinesin family member 5A; kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific; MY050; Neuronal kinesin heavy chain; NKHC1; NKHCspastic paraplegia 10 (autosomal dominant); SPG10 KIF5A ALS25, D12S1889, MY050, NEIMY, NKHC, SPG10 kinesin family member 5A kinesin heavy chain isoform 5A|KIF5A variant protein|kinesin heavy chain neuron-specific 1|kinesin, heavy chain, neuron-specific|neuronal kinesin heavy chain

For more info on KIF5A, check out the KIF5A Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KIF5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the KIF5A Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis