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Product Info Summary
| SKU: | A03635-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-KIF5A Antibody Picoband®
View all Kinesin 5A Antibodies
SKU/Catalog Number
A03635-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIF5A Antibody Picoband® catalog # A03635-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KIF5A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03635-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KIF5A recombinant protein (Position: E374-S1032).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03635-1 is reactive to KIF5A in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
117 kDa
Calculated molecular weight
28461 MW
Background of Kinesin 5A
Kinesin heavy chain isoform 5A is a protein that in humans is encoded by the KIF5A gene. This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03635-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, rat brain tissue, mouse brain tissue
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIF5A using anti-KIF5A antibody (A03635-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF5A antigen affinity purified polyclonal antibody (Catalog # A03635-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF5A at approximately 117 kDa. The expected band size for KIF5A is at 117 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-KIF5A antibody (A03635-1).
Overlay histogram showing K562 cells stained with A03635-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIF5A Antibody (A03635-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KIF5A (Source: Uniprot.org, NCBI)
Gene Name
KIF5A
Full Name
Kinesin heavy chain isoform 5A
Weight
28461 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Protein ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; LMAN1; ERGIC53; F5F8D KIF5A ALS25, D12S1889, MY050, NEIMY, NKHC, SPG10 kinesin family member 5A kinesin heavy chain isoform 5A|KIF5A variant protein|kinesin heavy chain neuron-specific 1|kinesin, heavy chain, neuron-specific|neuronal kinesin heavy chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF5A, check out the KIF5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIF5A Antibody Picoband® (A03635-1)
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