Product Info Summary
| SKU: | A07202 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Kin of IRRE-like protein 3 KIRREL3 Antibody
SKU/Catalog Number
A07202
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Kin of IRRE-like protein 3 KIRREL3 Antibody (Catalog # A07202). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Kin of IRRE-like protein 3 KIRREL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07202)
Host
Rabbit
Contents
KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3. The immunogen is located within amino acids 20 - 70 of KIRREL3.
Cross-reactivity
KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.
Reactive Species
A07202 is reactive to KIRREL3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
85.3 kDa
Background of KIRREL3
KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1). KIRREL3 is involved in the regulation of both glomerular and neural development (2), and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3). KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07202 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 5 μg/ml.
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Immunofluorescence of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 20 μg/ml.
Specific Publications For Anti-Kin of IRRE-like protein 3 KIRREL3 Antibody (A07202)
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