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Product Info Summary
| SKU: | M00689 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-KISS1 Antibody (N-Term)
SKU/Catalog Number
M00689
Size
80 µl
Description
Boster Bio Anti-KISS1 Antibody (N-Term) (Catalog # M00689). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KISS1 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00689)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This KISS1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 20-52 amino acids from human KISS1.
Reactive Species
M00689 is reactive to KISS1 in Human
Calculated molecular weight
14.7 kDa
Background of KISS1
Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00689 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-KISS1 Antibody (N-Term) at 1:2000 dilution
Lane 1: human placenta lysates
Lane 2: human skeletal muscle lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KISS1 Antibody (N-Term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysates
Lane 2: Jurkat whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-KISS1 Antibody (N-Term)at 1:500 dilution + SW480 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M00689 staining KISS1 in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Specific Publications For Anti-KISS1 Antibody (N-Term) (M00689)
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