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Product Info Summary
| SKU: | A30483 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-KLF Antibody
SKU/Catalog Number
A30483
Size
100ul
Form
Liquid
Description
Boster Bio Anti-KLF Antibody (Catalog# A30483). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30483)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human KLF. Immunogen sequence location: 291-340
Reactive Species
A30483 is reactive to KLF1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30483 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of VEC cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500.
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Western Blot analysis of various cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500.
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Western blot analysis of the lysates from K562 cells using KLF antibody.
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Western blot analysis of lysates from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-KLF Antibody (A30483)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-KLF Antibody
Question
I was wanting to use your anti-KLF antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
J. Parker
Verified customer
Asked: 2019-11-01
Answer
You can see on the product datasheet, A30483 anti-KLF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-KLF antibody (A30483)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-KLF antibody (A30483). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-KLF antibody A30483 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
B. Krishna
Verified customer
Asked: 2014-01-10
Answer
The anti-KLF antibody (A30483) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-01-10
