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Product Info Summary
| SKU: | M00120-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-KLF4 Antibody
SKU/Catalog Number
M00120-2
Size
50 µl
Description
Boster Bio Anti-KLF4 Antibody (Catalog # M00120-2). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KLF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00120-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2000CT726.79.22
Isotype
IgG1,κ
Immunogen
This KLF4 antibody is generated from a mouse immunized with a recombinant protein from the human region of human KLF4.
Reactive Species
M00120-2 is reactive to KLF4 in Human, Mouse
Calculated molecular weight
54.7 kDa
Background of KLF4
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00120-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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All lanes : Anti-KLF4 Antibody at 1:500-1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: NCCIT whole cell lysate
Lane 3: F9 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-KLF4 Antibody (M00120-2)
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