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Product Info Summary
| SKU: | M00120-2 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-KLF4 Antibody
SKU/Catalog Number
M00120-2
Size
50 µl
Description
Boster Bio Anti-KLF4 Antibody (Catalog # M00120-2). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-KLF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00120-2)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2000CT726.79.22
Isotype
IgG1,κ
Immunogen
This KLF4 antibody is generated from a mouse immunized with a recombinant protein from the human region of human KLF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00120-2 is reactive to KLF4 in Human, Mouse
Applications
M00120-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.671kDa
Background of KLF4
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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All lanes : Anti-KLF4 Antibody at 1:500-1:1000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: NCCIT whole cell lysate
Lane 3: F9 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 55 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54.671kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut) KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLF4 Antibody (M00120-2)
Hello CJ!
M00120-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deng S,Zhang Y,Wang Y,Lu X,Jiang Q.MicroRNA-92 regulates vascular smooth muscle cell function by targeting KLF4 during vascular restenosis and injury.Int J Clin Exp Pathol.2019 Dec 1;12(12):4253-4262.PMID:31933825;PMCID:PMC6949870.
Species: Human,Mouse
M00120-2 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:1000
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