Product Info Summary
SKU: | M00120 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-KLF4/Gklf Rabbit Monoclonal Antibody
SKU/Catalog Number
M00120
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-KLF4/Gklf Rabbit Monoclonal Antibody catalog # M00120. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLF4/Gklf Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00120)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AODA-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human KLF4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00120 is reactive to KLF4 in Human, Mouse, Rat
Applications
M00120 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa
Calculated molecular weight
54.671kDa
Background of KLF4
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of KLF4 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse liver, using KLF4 Antibody.
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Immunofluorescent analysis of Hela cells, using KLF4 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54.671kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut) KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLF4/Gklf Rabbit Monoclonal Antibody (M00120)
Hello CJ!
M00120 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deng S,Zhang Y,Wang Y,Lu X,Jiang Q.MicroRNA-92 regulates vascular smooth muscle cell function by targeting KLF4 during vascular restenosis and injury.Int J Clin Exp Pathol.2019 Dec 1;12(12):4253-4262.PMID:31933825;PMCID:PMC6949870.
Species: Human,Mouse
M00120 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:1:1000
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
M00120 usage in article: APP:WB, SAMPLE:MCF-7 CELL AND MDA-MB-231 CELL, DILUTION:NA
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
CHIR99021 enhances Klf4 Expression through ?-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-KLF4/Gklf Rabbit Monoclonal Antibody
Question
Is this M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody reactive to the isotypes of KLF4?
H. Jones
Verified customer
Asked: 2020-03-20
Answer
The immunogen of M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody is A synthesized peptide derived from human KLF4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-20
Question
We ordered your anti-KLF4/Gklf Rabbit Monoclonal antibody for WB on cervix carcinoma a few years ago. I am using mouse, and We want to use the antibody for ICC next. We want examining cervix carcinoma as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
I have checked the website and datasheets of our anti-KLF4/Gklf Rabbit Monoclonal antibody and it seems that M00120 has been tested on mouse in both WB and ICC. Thus M00120 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-31
Question
Can you help my question with product M00120, anti-KLF4/Gklf Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-17
Question
Do you have a BSA free version of anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 available?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-18
Question
My boss were content with the WB result of your anti-KLF4/Gklf Rabbit Monoclonal antibody. However we have been able to see positive staining in cervix lung nucleus. using this antibody. Is that expected? Could you tell me where is KLF4 supposed to be expressed?
D. Li
Verified customer
Asked: 2019-08-19
Answer
According to literature, cervix lung does express KLF4. Generally KLF4 expresses in nucleus. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-08-19
Question
I was wanting to use using your anti-KLF4/Gklf Rabbit Monoclonal antibody for pri-mirna transcription by rna polymerase ii studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We appreciate your inquiry. This M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody is tested on hela cells, mouse liver. It is guaranteed to work for IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-12
Question
I see that the anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 works with WB, what is the protocol used to produce the result images on the product page?
N. Kulkarni
Verified customer
Asked: 2019-06-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colonic mucosa using anti-KLF4/Gklf Rabbit Monoclonal antibody M00120. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
We are currently using anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
The anti-KLF4/Gklf Rabbit Monoclonal antibody (M00120) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-24
Question
My question regards to test anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 on mouse colonic mucosa for research purposes, then I may be interested in using anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
The products we sell, including anti-KLF4/Gklf Rabbit Monoclonal antibody M00120, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-07
Question
I was wanting to use your anti-KLF4/Gklf Rabbit Monoclonal antibody for WB for mouse colonic mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse colonic mucosa identification?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
It shows on the product datasheet, M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody has been tested for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colonic mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16
Question
See below the WB image, lot number and protocol we used for colonic mucosa using anti-KLF4/Gklf Rabbit Monoclonal antibody M00120. Please let me know if you require anything else.
B. Evans
Verified customer
Asked: 2017-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-05
Question
Will anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 work for WB with colonic mucosa?
A. Thomas
Verified customer
Asked: 2016-11-22
Answer
According to the expression profile of colonic mucosa, KLF4 is highly expressed in colonic mucosa. So, it is likely that anti-KLF4/Gklf Rabbit Monoclonal antibody M00120 will work for WB with colonic mucosa.
Boster Scientific Support
Answered: 2016-11-22
Question
Is a blocking peptide available for product anti-KLF4/Gklf Rabbit Monoclonal antibody (M00120)?
A. Krishna
Verified customer
Asked: 2016-07-13
Answer
We do provide the blocking peptide for product anti-KLF4/Gklf Rabbit Monoclonal antibody (M00120). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-07-13
Question
We have observed staining in mouse cervix lung. Are there any suggestions? Is anti-KLF4/Gklf Rabbit Monoclonal antibody supposed to stain cervix lung positively?
C. Williams
Verified customer
Asked: 2016-02-25
Answer
From literature cervix lung does express KLF4. From Uniprot.org, KLF4 is expressed in colonic mucosa, placenta, substantia nigra tongue, cervix lung, cervix carcinoma, liver, among other tissues. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-02-25
Question
Does M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Gonzalez
Verified customer
Asked: 2014-07-16
Answer
It shows on the product datasheet, M00120 anti-KLF4/Gklf Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-16